Ethanol Precipitation Protocol

Ethanol Precipitation Protocol

Overview

The method described in this protocol for precipitating sequenced product is derived from the ABI Ethanol Precipitation protocols. There are four basic steps:

  1. Combine the four sequenced products made from A, C, G, and T rxn mix into a nunc plate. The nunc plate is used because the circumference of its well allows the 384-split-well plate to fit nicely into it.
  2. Add the 95% ethanol and then mix well.
  3. Spin down the precipitated product and discard the supernatant.
  4. Allow the precipitated product to dry, then add deionized water to reconstitute it. Deionized water is used because it is less damaging to the 3700's capillaries than Formamide.

Equipment