Protocol for Preparing DNA Block

Overview

The protocol for preparing DNA outlines the procedures used to create:
  1. a master block at 5 ng/µL from the stock DNA we receive from Coriell Cell
    Repositories.
  2. a smaller working block from the master block.

Supplies


Setting up the Master Block

  1. Draw a line dividing the 96-well plate into halves.
  2. Make sure each of the halves contains an identical arrangement of DNA. (See Table 1). This arrangement makes it simple to transfer the DNA to a PCR plate in which two prime sets (each on one half of the plate) have been prepared for PCR.




Detailed Protocol

I. Dilute the sample

II. Pipette the DNA samples into the deep-well plate

III. Cover with oil and a mat; refrigerate

IV. Protocol for setting up the DNA working block